Plasmid transformation of Bacillus cereus on cellophane membranes
نویسندگان
چکیده
منابع مشابه
Cellophane Membranes
A cellophane membrane has proved a simple device for separating the L variant of Streptobacillus moniliformis from the bacterial form, as reported by P6rezMiravete and Calder6n (1956). When a culture was inoculated on cellophane resting on clear medium, they observed the development of the L type colonies only on the agar, whereas bacterial growth flourished on the surface of the cellophane. It...
متن کاملAntimicrobial Effects of Honey on Bacillus Cereus
Abstract Background and Objective: Honey is a healthy and nutritious food that has been used for a long time as a treatment for different diseases. One of the applied properties of honey is its antimicrobial effect, which differs between different types of honey due to variation of phenolic and antioxidant compositions. This study aimed to assess antimicrobial effect of honey on Bacillus cereu...
متن کاملEnhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA.
Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.
متن کاملTransformation of Bacillus polymyxa with plasmid DNA.
A plasmid transformation system was developed for Bacillus polymyxa ATCC 12321 and derivatives of this strain. The method utilizes a penicillin-treated-cell technique to facilitate uptake of the plasmid DNA. Low-frequency transformation (10(-6) per recipient cell) of plasmids pC194, pBD64, and pBC16 was accomplished with this method. Selection for the transformants was accomplished on both hype...
متن کاملTransformation of Bacillus cereus vegetative cells by electroporation.
Transformation of untreated vegetative cells of Bacillus cereus 569 with plasmid pC194 (1.8 megadaltons) by high-voltage electroporation resulted in a maximum of 2 x 10(-5) transformants per viable cell. Transformation of a 130-megadalton plasmid occurred at a comparable frequency. The method was simple, rapid, and yielded transformant colonies in 14 to 24 h. Transformation was obtained with un...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1990
ISSN: 0378-1097
DOI: 10.1016/0378-1097(90)90357-v